LAWSON DHTISSUE-48 Tissue Grinding is a special, fast, high-efficiency, multi-tube consistent system. It can extract and purify the original DNA, RNA and protein from any source (including soil, plant and animal tissues/organs, bacteria, yeast, fungi, spores, paleontological specimens, etc.).
Compared with other existing sample preparation methods, DHTISSUE-48 Tissue Grinding has the advantages of wide versatility, high efficiency and flexibility. The system avoids the laborious, time-consuming, inefficient and many other disadvantages of traditional methods such as grinding, homogenization, and ultrasonic treatment, and can efficiently, rapidly and stably lyse and purify nucleic acids and proteins of various types of samples.
Plant tissues: roots, stems, leaves, flowers, fruits, seeds, etc.
Animal tissues: brain, heart, lungs, stomach, liver, thymus, kidneys, intestines, lymph nodes, muscles, bones, etc.
Fungal bacteria: yeast, Escherichia coli, etc.
Food and Drugs: All kinds of food, pills, etc.
Volatile samples: coal, oil shale, wax products, etc.
Plastics, polymers: PE, PS, textiles, resins, etc.
DHTISSUE-48 Tissue Grinding adopts a special vertical up and down integrated vibration mode, through the high frequency reciprocating vibration, impact and shear of grinding beads (zirconia, steel balls, glass beads, ceramic beads). achieve goals quickly. The ground samples are more complete and uniform, the sample repeatability is better, and there is no cross-contamination between samples.
The number of operations is large, the effect is good, and the high-efficiency and fast work can complete the grinding of 2 × 24, 2 × 48, and 4 × 96 samples within 1 minute. Save time and effort, and the difference between batches and batches is small. The extracted protein has higher specific activity and longer nucleic acid fragments.
No cross-contamination The sample tube is completely closed during the crushing process, and disposable centrifuge tubes and beads can be used. The sample remains intact in the tube to avoid cross-contamination between samples and external contamination.
Easy to operate 1. Built-in program controller, which can set parameters such as grinding time and rotor vibration frequency;
2. Humanized operation interface.
Good stability 1. Vertical oscillation is adopted, the grinding is more sufficient and the stability is better;
2. During the operation of the instrument, the noise is less than 55dB, which will not interfere with other experiments or instruments.
Convenient for low-temperature operation When a low-temperature grinding environment is required, the adapter with the sample can be immersed in liquid nitrogen to cool for 1-2 minutes. After taking it out, move it to the host and quickly fix it to start grinding without re-freezing, saving liquid nitrogen .
Good reproducibility Set the same procedure for the same tissue sample to obtain the same grinding effect. The working time is short and the sample temperature does not rise.
Applications Tissue grinding, homogenization, cell disruption, homogenization, material dispersion, preparation, sample mixing, shaking, etc.
Throughput Maximum throughput in 15 seconds 24 samples can be processed at the same time, including liquid nitrogen freezing adapters for 12 and 24 positions
Compatible sample book 48*(0.2-0.5ML) /48*2ML / 24*5ML /12*(7-15)ML /2*25ML /2*50ML, can be customized for grinding of various specifications Tube
Display mode LCD screen display, touch screen display (upgradable)
Anti-vibration principle German patent schleifen-1 working method Patented anti-vibration principle, and three-dimensional integrated movement method of up and down and left and right shaking The patented grinding bead movement method ensures maximum sample processing and instant crushing effect.
Maximum feed size no requirement, adjust according to adapter
Final output particle size 5µm
Number of grinding platforms (Number of acceptable grinding jars)>2
Fastening device with automatic centering
Homogenization speed 0～70 Hz/sec, working time: 0sec～99min, user can set by himself
Grinding ball diameter 0.1～30mm
Grinding ball material alloy steel, chrome steel, zirconia, tungsten carbide, quartz sand
Accelerate Maximum speed in 2 seconds
Slow Reach minimum speed within 2 seconds
Noise level <55db
Grinding method Wet grinding, dry grinding, low temperature grinding can be
The constant temperature can be reconfigured with the ability to upgrade to ultra-low temperature liquid nitrogen refrigeration or air cooler refrigeration
Adapter material PTFE, alloy steel
Safety instructions Fastening device with automatic centering Safety lock while working, full protection
Grinding Kit Materials Hard Rigid, , Teflon (Teflon), Zirconia
Weight 30 Kg
A host DHTISSUE-48
Adapter Ø2ml set
Refrigeration adapter a set of Ø2ml or a set of Ø1.5ml (choose 1 from 2)
Grinding Beads 1. Ø5 imported stainless steel grinding beads 1000 or Ø5 imported ceramic grinding beads 1000 (choose 1 from 2);
2. Ø3 imported stainless steel grinding beads 1000.
Dedicated grinding tube 200
Dedicated bead gun a set (optional)
Animal tissue-DNA LW-D-P1 (optional)
Extraction object: animal tissue-RNA LW-D-P2 (optional)
Extraction object: animal tissue-protein LW-D-P3 (optional)
Extraction object: plant tissue-DNA LW-Z-P1 (optional)
Extraction object: plant tissue-RNA LW-Z-P2 (optional)
Extraction object: plant tissue-protein LW-Z-P3 (optional)
Extraction object: Microorganism-DNA LW-W-P1 (optional)
Extraction object: Microorganism-RNA LW-W-P2 (optional)
Extraction object: Microorganism- Protein LW-W-P3 (optional)
Precautions before use:
1. The matching LW kit can be used to quickly, efficiently, stably, and batch extract DNA/RNA proteins from samples.
2. 1Hz=30 RPM / 1Hz=100RPM
3. . Conventional adapters can be placed in the refrigerator to give the sample a cold environment while working. It can be equipped with an adapter with refrigeration, which can be soaked in liquid nitrogen for more than 30 minutes.
4. The noise emission value for the working environment depends on the type of sample and the settings of the grinder. The parameters in the table are in the no-load state.
5. . Small samples typically used for DNA and RNA isolation can be prepared in disposable centrifuge tubes. Adapters made of teflon material can be used to hold 5 or 10 disposable sample tubes for cell disruption. In multi-sample tissue Cells and tissues in the grinder are rapidly and efficiently broken up in a very short time, thus eliminating the need for additional cryogenic refrigeration.